2,3-dinor-TXB2/2,3-dinor Thromboxane B2 ELISA Kit
The 2,3-dinor-TXB2 / 2,3-dinor Thromboxane B2 ELISA Kit can assay for 2,3-dinor-TXB2 / 2,3-dinor Thromboxane B2 in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.
How our 2,3-dinor-TXB2 / 2,3-dinor Thromboxane B2 ELISA Kits Work?
The ELISA Genie ELISA (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today’s scientists demand high quality consistent data for high impact journals, We have developed a range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands.
This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 2,3-dinor-TXB2 / 2,3-dinor Thromboxane B2 . During the reaction, 2,3-dinor-TXB2 / 2,3-dinor Thromboxane B2 in the sample or standard competes with a fixed amount of 2,3-dinor-TXB2 / 2,3-dinor Thromboxane B2 on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to 2,3-dinor-TXB2 / 2,3-dinor Thromboxane B2 . Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 2,3-dinor-TXB2 / 2,3-dinor Thromboxane B2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Product name:
23-dinor-TXB2 (23-dinor Thromboxane B2) ELISA Kit
- Catalogue No.:
23-dinor-TXB2(23-dinor Thromboxane B2)
- Detection method:
Competitive ELISA Coated with Antigen
This immunoassay kit allows for the in vitro quantitative determination of 23-dinor-TXB2 concentrations in serum plasma and other biological fluids.
4 „ƒ for 6 months
Matrices listed below were spiked with certain level of 23-dinor-TXB2 and the recovery rates were calculated by comparing the measured value to the expected amount of 23-dinor-TXB2 in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 85-104 93 EDTA plasma(n=5) 95-104 100 UFH plasma(n=5) 86-99 93
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 23-dinor-TXB2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16 serum(n=5) 89-105% 88-105% 87-96% 87-105% EDTA plasma(n=5) 83-96% 83-101% 85-97% 86-97% UFH plasma(n=5) 85-100% 84-96% 83-99% 80-98%
For Research Use Only