6-keto-PGF1a/6-keto-prostaglandin F1a ELISA Kit
The 6-keto-PGF1a / 6-keto-prostaglandin F1a ELISA Kit can assay for 6-keto-PGF1a / 6-keto-prostaglandin F1a in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.
How our 6-keto-PGF1a / 6-keto-prostaglandin F1a ELISA Kits Work?
The ELISA Genie ELISA (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today’s scientists demand high quality consistent data for high impact journals, We have developed a range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands.
This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 6-keto-PGF1a / 6-keto-prostaglandin F1a . During the reaction, 6-keto-PGF1a / 6-keto-prostaglandin F1a in the sample or standard competes with a fixed amount of 6-keto-PGF1a / 6-keto-prostaglandin F1a on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to 6-keto-PGF1a / 6-keto-prostaglandin F1a . Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 6-keto-PGF1a / 6-keto-prostaglandin F1a in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Product name:
6-keto-PGF1a (6-keto-prostaglandin F1a) ELISA Kit
- Catalogue No.:
- Detection method:
Competitive ELISA Coated with Antigen
This immunoassay kit allows for the in vitro quantitative determination of 6-keto-PGF1a concentrations in serum plasma and other biological fluids.
4 „ƒ for 6 months
Matrices listed below were spiked with certain level of 6-keto-PGF1a and the recovery rates were calculated by comparing the measured value to the expected amount of 6-keto-PGF1a in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 87-102 95 EDTA plasma(n=5) 89-101 95 UFH plasma(n=5) 87-102 96
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 6-keto-PGF1a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16 serum(n=5) 90-98% 93-105% 87-96% 88-105% EDTA plasma(n=5) 83-99% 85-99% 84-95% 83-94% UFH plasma(n=5) 84-100% 83-100% 81-93% 82-98%
For Research Use Only