8-epi-PGF2 alpha/8-Epi-Prostaglandin F2 Alpha ELISA Kit
The 8-epi-PGF2alpha / 8-Epi-Prostaglandin F2 Alpha ELISA Kit can assay for 8-epi-PGF2alpha / 8-Epi-Prostaglandin F2 Alpha in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.
How our 8-epi-PGF2alpha / 8-Epi-Prostaglandin F2 Alpha ELISA Kits Work?
The ELISA Genie ELISA (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today’s scientists demand high quality consistent data for high impact journals, We have developed a range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands.
This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 8-epi-PGF2alpha / 8-Epi-Prostaglandin F2 Alpha. During the reaction, 8-epi-PGF2alpha / 8-Epi-Prostaglandin F2 Alpha in the sample or standard competes with a fixed amount of 8-epi-PGF2alpha / 8-Epi-Prostaglandin F2 Alpha on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to 8-epi-PGF2alpha / 8-Epi-Prostaglandin F2 Alpha. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 8-epi-PGF2alpha / 8-Epi-Prostaglandin F2 Alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Product name:
8-epi-PGF2alpha(8-Epi-Prostaglandin F2 Alpha) ELISA Kit
- Catalogue No.:
8-epi-PGF2alpha(8-Epi-Prostaglandin F2 Alpha)
- Detection method:
Competitive ELISA Coated with Antigen
This immunoassay kit allows for the in vitro quantitative determination of 8-epi-PGF2alpha concentrations in serum plasma and other biological fluids.
4 „ƒ for 6 months
Matrices listed below were spiked with certain level of 8-epi-PGF2alpha and the recovery rates were calculated by comparing the measured value to the expected amount of 8-epi-PGF2alpha in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 90-99 95 EDTA plasma(n=5) 85-101 94 UFH plasma(n=5) 86-95 91
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 8-epi-PGF2alpha and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16 serum(n=5) 94-100% 92-104% 85-94% 91-102% EDTA plasma(n=5) 87-94% 90-101% 85-100% 86-98% UFH plasma(n=5) 89-100% 85-98% 80-100% 82-96%
For Research Use Only