Capturem™ His-Tagged Purification Miniprep Kit
Capturem nickel-functionalized membranes for his-tagged purification are a revolutionary solution for recombinant protein purification that effortlessly outperforms conventional methods. Novel nylon membranes have been assembled into spin columns and filtration devices, creating no-waiting, room-temperature workflows (just 5–30 minutes from loading a sample through elution) compatible with both mammalian and bacterial cells. Exceptional performance is consistent under a wide range of research settings, including denaturing conditions, in the presence of additives (e.g., BME, EDTA, DTT, glycerol, TCEP, etc.), and when performing tandem purification of his-tagged proteins.
The modified nylon membranes have a small bed volume, yet still produce high-purity, concentrated protein, due to the chemically enhanced surface area of the pores. With this technology, protein-binding capacity is comparable to, or better than, resins that bind 75 mg or more per cm3 of membrane. Miniprep, maxiprep, 24-well-plate, 96-well-plate, and large-volume formats are available to accommodate a range of input volumes. Furthermore, the short residence time of protein samples greatly reduces the possibility of protein degradation or loss of activity, giving downstream applications a higher probability of success.
- No-waiting workflow—5-minute, room-temperature protocol
- High purity and yield—small column bed volumes trap fewer contaminants and allow large-volume washes
- Compatible with wide range of conditions—native and denaturing conditions; with common additives (e.g., EDTA, DTT, BME, glycerol, TCEP, etc.)
- Lysis buffer compatibility—xTractor Buffer; buffers from other vendors
- Different cell systems—easy purification from mammalian and bacterial cells
- Biochemical/enzymatic assays
- Pull-down or other protein interaction assays
- Confirmation screening
- Protein/ligand affinity analysis
- Crystallography or other structural analyses
- Antibody production
- Toxicology studies
- Protein transfer to live cells