EasyPGX® ready EGFR cat.no. RT023 (48 test, CE IVD)
Features
Detection of the main mutations of exons 2 (condosn 12, 13) of exon 3 (condon 59, 61) and the exon 4 (condons 117, 146) of NRAS gene using 8 oligo mixes. Each mix allows the co-amplification of one or more mutated alleles plus an endogenous control gene. A specific oligo control mix enables the evaluation of the quality and the quantity of the DNA in each sample.
Controls
The kit is provided with the following controls:
Sensitivity
The kit allows the detection of low percentages of mutated allele in presence of high amounts of wild-type genomic DNA by real-time amplification with sequence-specific probes marked with FAMb and HEX. LOD down to 0.5%
Starting material
The kit allows the analysis of DNA extracted from fresh, frozen formalin-fixed parafin in-embedded (FFPE) tissues and plasma.
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