BAD (EP315) Rabbit Monoclonal Antibody
BAD (EP315) Rabbit Monoclonal Antibody (Bcl2-associated agonist of cell death), initially identified in a yeast two hybrid screen interacting with Bcl-2 and Bcl-XL, is a pro-apoptotic member of the Bcl-2 family. It is a unique BH3-only family member primarily regulated through phosphorylation at conserved serine residues. BAD is activated upon dephosphorylation, interacts with Bcl-2 and Bcl-XL to ablate their pro-survival function, and induces downstream activators (Bax and Bak) to activate cellular apoptosis. BAD is cytoplasmic localized and broadly expressed in human tissues, including the skin, heart, lungs, stomach, colon, liver, kidney, and pancreas. Loss of BAD protein has been reported to contribute to tumorigenesis and chemotherapy resistance. Loss-of-function models are associated with increased incidence of tumors, and increased death rates from tumors. BAD-/- breast cancer cell lines are chemoresistant, and BAD-deficient mice develop diffuse large B-cell lymphoma. Furthermore, BAD expression has been studied in prostate cancer, non-small cell lung carcinoma (NSCLC), and hepatocellular carcinomas. Decreased BAD expression was reported in 50% of hepatocellular carcinomas, and loss of BAD was shown to be an independent marker of poor prognosis in NSCLC patients.
- Dilution Range: 1:100 – 1:200*