F-E3/Free Estriol ELISA Kit
The F-E3 / Free Estriol ELISA Kit can assay for F-E3 / Free Estriol in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.
How our F-E3 / Free Estriol ELISA Kits Work?
The ELISA Genie ELISA (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today’s scientists demand high quality consistent data for high impact journals, We have developed a range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands.
This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with F-E3 / Free Estriol. During the reaction, F-E3 / Free Estriol in the sample or standard competes with a fixed amount of F-E3 / Free Estriol on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to F-E3 / Free Estriol. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of F-E3 / Free Estriol in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Product name:
F-E3(Free Estriol) ELISA Kit
- Catalogue No.:
- Detection method:
Competitive ELISA Coated with Antigen
This immunoassay kit allows for the in vitro quantitative determination of F-E3 concentrations in serum plasma and other biological fluids.
4 „ƒ for 6 months
Matrices listed below were spiked with certain level of F-E3 and the recovery rates were calculated by comparing the measured value to the expected amount of F-E3 in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 85-98 93 EDTA plasma(n=5) 85-102 98 UFH plasma(n=5) 86-101 92
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of F-E3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16 serum(n=5) 87-105% 97-101% 86-96% 85-101% EDTA plasma(n=5) 87-101% 88-97% 82-101% 83-96% UFH plasma(n=5) 85-100% 95-99% 82-99% 83-99%
For Research Use Only