Human 12-HETE ELISA Kit
The Human 12-HETE ELISA Kit can assay for 12-HETE in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.
How our 12-HETE ELISA Kits Work?
The ELISA Genie ELISA (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today’s scientists demand high quality consistent data for high impact journals, We have developed a range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands.
This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 12-HETE. During the reaction, 12-HETE in the sample or standard competes with a fixed amount of 12-HETE on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to 12-HETE. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 12-HETE in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Detection method:
Competitive ELISA Coated with Antigen
This immunoassay kit allows for the in vitro quantitative determination of 12-HETE concentrations in serum plasma and other biological fluids.
4’C for 6 months
Matrices listed below were spiked with certain level of 12-HETE and the recovery rates were calculated by comparing the measured value to the expected amount of 12-HETE in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 85-102 95 EDTA plasma(n=5) 86-105 96 UFH plasma(n=5) 90-101 96
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 12-HETE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16 serum(n=5) 89-102% 86-104% 89-102% 85-104% EDTA plasma(n=5) 83-89% 88-97% 91-101% 84-100% UFH plasma(n=5) 83-91% 80-97% 80-100% 82-98%
For Research Use Only