Human ATF2 / Activating Transcription Factor 2 ELISA Kit
The ELISA Genie ATF2 / Activating Transcription Factor 2 ELISA Kit can assay for ATF2 / Activating Transcription Factor 2 in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.
How our ATF2 / Activating Transcription Factor 2 ELISA Kits Work?
The ELISA Genie (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today’s scientists demand high quality consistent data for high impact journals, ELISA Genie have developed our range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands. Our assay kits use a quantitative sandwich ELISA technique and each kit comes with highly specific antibodies pre-coated onto a 96-well microtiter plate.
At ELISA Genie we understand the need for speed! Therefore, we have developed an ultra-fast protocol meaning you achieve your results rapidly. So, once you have prepared and plated your samples, blanks and standards, you simply incubate with a highly specific biotin-conjugated primary antibody and Avidin conjugated to Horseradish Peroxidase (HRP) and incubate for the appropriate length of time. After washing the plate according to the protocol and addition of the TMB (3,3′,5,5′-Tetramethylbenzidine) solution, the appearance of a blue colour should be detected due to an enzymatic reaction catalysed by HRP. Next step is the addition of the Stop Solution which terminates the HRP reaction and the blue colour turns yellow with the signal intensity measured on a plate reader at 450nm. The amount of bound ATF2 / Activating Transcription Factor 2 is proportional to the signal generated by the reaction meaning the kit assay gives you a quantitative measurement of the analyte in your samples.
Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5′-TGACGTCA-3′) or to AP-1 (activator protein 1) consensus sequences (5′-TGACTCA-3′). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro. In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type.
- Post-Translational Modification:
Phosphorylation of Thr-69 by MAPK14 and MAPK11, and at Thr-71 by MAPK1/ERK2, MAPK3/ERK1, MAPK11, MAPK12 and MAPK14 in response to external stimulus like insulin causes increased transcriptional activity. Phosphorylated by PLK3 following hyperosmotic stress. Also phosphorylated and activated by JNK and CaMK4. ATM-mediated phosphorylation at Ser-490 and Ser-498 stimulates its function in DNA damage response. Phosphorylation at Ser-62, Thr-73 and Ser-121 activates its transcriptional activity. Phosphorylation at Thr-69 or Thr-71 enhances its histone acetyltransferase (HAT) activity.
- Uniprot ID: P15336
- Detection method:
Sandwich ELISA Double Antibody
This immunoassay kit allows for the in vitro quantitative determination of ATF-2 concentrations in serum plasma and other biological fluids.
4’C for 6 months
Matrices listed below were spiked with certain level of ATF-2 and the recovery rates were calculated by comparing the measured value to the expected amount of ATF-2 in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 85-102 93 EDTA plasma(n=5) 87-99 94 UFH plasma(n=5) 86-104 95
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ATF-2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16 serum(n=5) 85-105% 87-102% 92-105% 87-96% EDTA plasma(n=5) 85-100% 82-99% 83-101% 83-100% UFH plasma(n=5) 88-100% 83-100% 82-100% 81-100%
For Research Use Only