Human Brain, Hippocampus QUICK-Clone™ cDNA
A high-quality cDNA template is necessary to obtain good results from PCR amplification. QUICK-Clone cDNA is premade double-stranded cDNA from which you can amplify sequences of interest using gene-specific primers, and is ideal for amplifying previously isolated, structurally related, or cross-species cDNAs. Synthesized from premium, high-quality poly A+ RNA from human brain and central nervous system tissues using an oligo(dT) primer, QUICK-Clone cDNA is purified to remove residual RNA and size-selected to eliminate cDNA fragments smaller than 400 bp. QUICK-Clone cDNA allows you to amplify cDNAs of interest while avoiding traditional library construction and screening steps, and can also be used to generate hybridization probes using gene-specific or degenerate primers (Lee et al. 1988; Parmentier et al. 1989; Schuchman, Jackson, and Desnick 1990; Vallins et al. 1990; Wilks et al. 1989).
Highly purified, double-stranded cDNA
Clone genes directly by PCR, rather than library screening
Prepared from high-quality human tissues
Ideal for amplifying previously isolated, structurally related, or cross-species cDNAs
Size: 2 x 10 Rxns
Clone cDNAs without library screening
Generate hybridization probes using gene-specific or degenerate primers
- Ideal for amplifying previously isolated, structurally related, or cross-species cDNAs
Lee, C. C. et al. Generation of cDNA probes directed by amino acid sequence: cloning of urate oxidase. Science239, 1288–91 (1988).
Parmentier, M. et al. Molecular cloning of the thyrotropin receptor. Science246, 1620–2 (1989).
Schuchman, E. H., Jackson, C. E. & Desnick, R. J. Human arylsulfatase B: MOPAC cloning, nucleotide sequence of a full-length cDNA, and regions of amino acid identity with arylsulfatases A and C. Genomics6, 149–58 (1990).
Vallins, W. J. et al. Molecular cloning of human cardiac troponin I using polymerase chain reaction. FEBS Lett.270, 57–61 (1990).
Wilks, A. F., Kurban, R. R., Hovens, C. M. & Ralph, S. J. The application of the polymerase chain reaction to cloning members of the protein tyrosine kinase family. Gene85, 67–74 (1989).