Human Gasdermin-C (GSDMC) ELISA Kit
The ELISA Genie Human Gasdermin-C (GSDMC) ELISA Kit can assay for Human Gasdermin-C in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.
How do our Human Gasdermin-C (GSDMC) ELISA Kits work?
The ELISA Genie Human Gasdermin-C (GSDMC) ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Gasdermin-C. During the reaction, in the sample or standard competes with a fixed amount of biotin-labeled for sites on a pre-coated Monoclonal antibody specific to Human Gasdermin-C. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Gasdermin-C, Melanoma-derived leucine zipper-containing extranuclear factor, GSDMC, MLZE