Human IL-2 ELISA MAX™ Standard 5 Plates
- Reactivity
- Human
- Application
ELISA
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- Application References
(PubMed link indicates BioLegend citation)
- Sautto GA, et al. 2015. Gut. PubMed
- Sautto G, et al. 2015. Gut. . PubMed
- Isernhagen A, et al. 2015. EMBO Mol Med. 7: 1480 – 1502. PubMed
- Grech D, et al. 2016. J Acupunct Meridian Stud. 9:234-241. PubMed
- Standard Range
- 7.8-500 pg/mL
- Materials Not Included
- Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
- A microplate reader capable of measuring absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
- Deionized (DI) water
- Coating Buffer: 8.4 g NaHCO3, 3.56 g Na2CO3, add DI H2O to 1.0 L, pH to 9.5 (BioLegend Cat. No. 421701 is recommended.)
- Assay Diluent: 10% Fetal Bovine Serum or 1% BSA in Phosphate-Buffered Saline (PBS) (BioLegend Cat. No. 421203 is recommended.)
- PBS: 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add DI water to 1.0 L, pH to 7.4
- Wash Buffer: Phosphate-Buffered Saline (PBS) + 0.05% Tween-20 (BioLegend Cat. No. 421601 is recommended.)
- Wash bottle or automated microplate washer
- TMB Substrate Solution – BioLegend Cat. No. 421101 is recommended.
- Stop Solution (2 N H2SO4)
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Absorbent paper
- Kit Contents
- Human IL-2 ELISA MAX™ Capture Antibody (200X)
- Human IL-2 ELISA MAX™ Detection Antibody (200X)
- Human IL-2 Standard
- Avidin-HRP (1000X)
- Cell Sources
- T cells
- Gene ID
- 3558View all products for this Gene ID
- UniProt
- View information about IL-2 on UniProt.org
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