Human IL-31 ELISA MAX™ Deluxe
Interleukin 31 (IL-31) is a newly identified member of the gp130/IL-6 cytokine family that is composed of 144 amino acid and has molecular mass of 16 kD. IL-31 is produced mainly by T helper type 2 cells. The mature form of the protein contains a four-helix structure. Human IL-31 shares 31% amino acid sequence identity with mouse IL-31.
The BioLegend ELISA MAX™ Deluxe Set is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). A human IL-31 specific goat polyclonal antibody is first coated on a 96-well plate. Standards and samples are added to the wells, and IL-31 binds to the immobilized capture antibody. Next, a biotinylated goat polyclonal anti-human IL-31 detection antibody is added, producing an antibody-antigen-antibody “sandwich”. Avidin horseradish peroxidase is subsequently added, followed by TMB Substrate Solution, producing a blue color in proportion to the concentration of IL-31 present in the sample. Finally, the Stop Solution changes the reaction color from blue to yellow, and the microwell absorbance is read at 450 nm with a microplate reader.
- Reactivity
- Human
- Application
ELISA
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- Sensitivity
- 0.6 pg/mL
- Standard Range
- 37.5-2,400 pg/mL
- Materials Not Included
- PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4,
0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm
filtered - Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS +
0.05% Tween-20 - Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid
solution, e.g. 2N H2SO4 - Plate Sealers: BioLegend Cat. No. 423601 is recommended
- Deionized (DI) water
- A microplate reader capable of measuring absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Absorbent paper
- PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4,
- Kit Contents
- Human IL-31 ELISA MAX™ Capture Antibody (200X)
- Human IL-31 ELISA MAX™ Detection Antibody (200X)
- Human IL-31 Standard
- Avidin-HRP (1000X)
- Substrate Solution C
- Coating Buffer B (5X)
- Assay Diluent A (5X)
- NUNC Maxisorp™ 96 MicroWell Plates
- Lot-Specific Certificate of Analysis/ELISA MAX™ Deluxe Set Protocol
- Cell Sources
- Helper T Cell
- Gene ID
- 386653View all products for this Gene ID
- UniProt
- View information about IL-31 on UniProt.org
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