Human PMAIP1 / NOXA ELISA Kit
The ELISA Genie PMAIP1 / NOXA ELISA Kit can assay for PMAIP1 / NOXA in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.
How our PMAIP1 / NOXA ELISA Kits Work?
The ELISA Genie (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today’s scientists demand high quality consistent data for high impact journals, ELISA Genie have developed our range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands. Our assay kits use a quantitative sandwich ELISA technique and each kit comes with highly specific antibodies pre-coated onto a 96-well microtiter plate.
At ELISA Genie we understand the need for speed! Therefore, we have developed an ultra-fast protocol meaning you achieve your results rapidly. So, once you have prepared and plated your samples, blanks and standards, you simply incubate with a highly specific biotin-conjugated primary antibody and Avidin conjugated to Horseradish Peroxidase (HRP) and incubate for the appropriate length of time. After washing the plate according to the protocol and addition of the TMB (3,3′,5,5′-Tetramethylbenzidine) solution, the appearance of a blue colour should be detected due to an enzymatic reaction catalysed by HRP. Next step is the addition of the Stop Solution which terminates the HRP reaction and the blue colour turns yellow with the signal intensity measured on a plate reader at 450nm. The amount of bound PMAIP1 / NOXA is proportional to the signal generated by the reaction meaning the kit assay gives you a quantitative measurement of the analyte in your samples.
Promotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53/TP53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1. Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1.
- Post-Translational Modification:
- Uniprot ID: Q13794
PMAIP1/PMA-induced protein 1/Phorbol-12-myristate-13-acetate-induced protein 1/NOXA/Immediate-early-response protein APR/Protein Noxa
- Detection method:
Sandwich ELISA Double Antibody
This immunoassay kit allows for the in vitro quantitative determination of PMAIP1 concentrations in serum plasma and other biological fluids.
4’C for 6 months
Matrices listed below were spiked with certain level of PMAIP1 and the recovery rates were calculated by comparing the measured value to the expected amount of PMAIP1 in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 87-102 93 EDTA plasma(n=5) 85-98 90 UFH plasma(n=5) 91-101 98
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PMAIP1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16 serum(n=5) 88-104% 92-105% 86-102% 91-103% EDTA plasma(n=5) 84-99% 95-101% 86-99% 82-100% UFH plasma(n=5) 80-92% 82-95% 86-95% 84-97%
For Research Use Only