Human SMAD2 ELISA Kit
The ELISA Genie SMAD2 ELISA Kit can assay for SMAD2 in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.
How our SMAD2 ELISA Kits Work?
The ELISA Genie (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today’s scientists demand high quality consistent data for high impact journals, ELISA Genie have developed our range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands. Our assay kits use a quantitative sandwich ELISA technique and each kit comes with highly specific antibodies pre-coated onto a 96-well microtiter plate.
At ELISA Genie we understand the need for speed! Therefore, we have developed an ultra-fast protocol meaning you achieve your results rapidly. So, once you have prepared and plated your samples, blanks and standards, you simply incubate with a highly specific biotin-conjugated primary antibody and Avidin conjugated to Horseradish Peroxidase (HRP) and incubate for the appropriate length of time. After washing the plate according to the protocol and addition of the TMB (3,3′,5,5′-Tetramethylbenzidine) solution, the appearance of a blue colour should be detected due to an enzymatic reaction catalysed by HRP. Next step is the addition of the Stop Solution which terminates the HRP reaction and the blue colour turns yellow with the signal intensity measured on a plate reader at 450nm. The amount of bound SMAD2 is proportional to the signal generated by the reaction meaning the kit assay gives you a quantitative measurement of the analyte in your samples.
- Description:
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
- Post-Translational Modification:
Phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. TGF-beta-induced Ser-465/467 phosphorylation declines progressively in a KMT5A-dependent manner. Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin. Phosphorylated by PDPK1. In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation. Monoubiquitinated, leading to prevent DNA-binding. Deubiquitination by USP15 alleviates inhibition and promotes activation of TGF-beta target genes (PubMed:21947082). Ubiquitinated by RNF111, leading to its degradation: only SMAD2 proteins that are ‘in use’ are targeted by RNF111, RNF111 playing a key role in activating SMAD2 and regulating its turnover. Acetylated on Lys-19 by coactivators in response to TGF-beta signaling, which increases transcriptional activity. Isoform short: Acetylation increases DNA binding activity in vitro and enhances its association with target promoters in vivo. Acetylation in the nucleus by EP300 is enhanced by TGF-beta.
- Uniprot ID: Q15796
- Alias:
SMAD2/JV18/MADH2/MADR2/JV18-1/hMAD-2/hSMAD2/SMAD family member 2(SMAD 2/Smad2/hSMAD2)/JV18-1/Mad-related protein 2(hMAD-2)/
- Detection method:
Sandwich ELISA Double Antibody
- Application:
This immunoassay kit allows for the in vitro quantitative determination of SMAD2 concentrations in serum plasma and other biological fluids.
- Size:
96T
- Range:
0.312-20ng/ml
- Sensitivity:
< 0.188ng/ml
- Storage:
4’C for 6 months
- Recovery:
Matrices listed below were spiked with certain level of SMAD2 and the recovery rates were calculated by comparing the measured value to the expected amount of SMAD2 in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 88-104 96 EDTA plasma(n=5) 90-100 94 UFH plasma(n=5) 88-101 95 - Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SMAD2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16 serum(n=5) 86-103% 89-103% 85-97% 91-105% EDTA plasma(n=5) 83-99% 88-101% 82-100% 82-93% UFH plasma(n=5) 81-99% 85-92% 80-98% 80-99% - CV(%):
Intra-Assay: CV<8%
Inter-Assay: CV<10% - Note:
For Research Use Only
Reactivity | Human |
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ELISA Type | Sandwich |
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