Human TFCP2 / Alpha-globin transcription factor CP2 ELISA Kit
The ELISA Genie TFCP2 / Alpha-globin transcription factor CP2 ELISA Kit can assay for TFCP2 / Alpha-globin transcription factor CP2 in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.
How our TFCP2 / Alpha-globin transcription factor CP2 ELISA Kits Work?
The ELISA Genie (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today’s scientists demand high quality consistent data for high impact journals, ELISA Genie have developed our range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands. Our assay kits use a quantitative sandwich ELISA technique and each kit comes with highly specific antibodies pre-coated onto a 96-well microtiter plate.
At ELISA Genie we understand the need for speed! Therefore, we have developed an ultra-fast protocol meaning you achieve your results rapidly. So, once you have prepared and plated your samples, blanks and standards, you simply incubate with a highly specific biotin-conjugated primary antibody and Avidin conjugated to Horseradish Peroxidase (HRP) and incubate for the appropriate length of time. After washing the plate according to the protocol and addition of the TMB (3,3′,5,5′-Tetramethylbenzidine) solution, the appearance of a blue colour should be detected due to an enzymatic reaction catalysed by HRP. Next step is the addition of the Stop Solution which terminates the HRP reaction and the blue colour turns yellow with the signal intensity measured on a plate reader at 450nm. The amount of bound TFCP2 / Alpha-globin transcription factor CP2 is proportional to the signal generated by the reaction meaning the kit assay gives you a quantitative measurement of the analyte in your samples.
Binds a variety of cellular and viral promoters including fibrinogen, alpha-globin, SV40 and HIV-1 promoters. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction with UBP1. Functions as part of the SSP (stage selector protein) complex. Facilitates the interaction of the gamma-globin genes with enhancer elements contained in the locus control region in fetal erythroid cells. Interacts by binding to the stage selector element (SSE) in the proximal gamma-globin promoter.
- Post-Translational Modification:
- Uniprot ID: Q12800
TFCP2/Alpha-globin transcription factor CP2/SAA3 enhancer factor/Transcription factor LSF/LSF/SEF/LBP1C/LSF1D/TFCP2C
- Detection method:
Sandwich ELISA Double Antibody
This immunoassay kit allows for the in vitro quantitative determination of TFCP2 concentrations in serum plasma and other biological fluids.
4’C for 6 months
Matrices listed below were spiked with certain level of TFCP2 and the recovery rates were calculated by comparing the measured value to the expected amount of TFCP2 in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 87-99 93 EDTA plasma(n=5) 86-101 95 UFH plasma(n=5) 88-104 95
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TFCP2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16 serum(n=5) 86-100% 93-100% 86-98% 92-105% EDTA plasma(n=5) 83-92% 82-100% 89-95% 84-100% UFH plasma(n=5) 83-98% 84-99% 85-100% 86-100%
For Research Use Only