Human VEGF A / Vascular Endothelial Cell Growth Factor A ELISA Kit
The ELISA Genie VEGF A / Vascular Endothelial Cell Growth Factor A ELISA Kit can assay for VEGF A / Vascular Endothelial Cell Growth Factor A in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.
How our VEGF A / Vascular Endothelial Cell Growth Factor A ELISA Kits Work?
The ELISA Genie (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today’s scientists demand high quality consistent data for high impact journals, ELISA Genie have developed our range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands. Our assay kits use a quantitative sandwich ELISA technique and each kit comes with highly specific antibodies pre-coated onto a 96-well microtiter plate.
At ELISA Genie we understand the need for speed! Therefore, we have developed an ultra-fast protocol meaning you achieve your results rapidly. So, once you have prepared and plated your samples, blanks and standards, you simply incubate with a highly specific biotin-conjugated primary antibody and Avidin conjugated to Horseradish Peroxidase (HRP) and incubate for the appropriate length of time. After washing the plate according to the protocol and addition of the TMB (3,3′,5,5′-Tetramethylbenzidine) solution, the appearance of a blue colour should be detected due to an enzymatic reaction catalysed by HRP. Next step is the addition of the Stop Solution which terminates the HRP reaction and the blue colour turns yellow with the signal intensity measured on a plate reader at 450nm. The amount of bound VEGF A / Vascular Endothelial Cell Growth Factor A is proportional to the signal generated by the reaction meaning the kit assay gives you a quantitative measurement of the analyte in your samples.
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and UFH. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development.
- Post-Translational Modification:
- Uniprot ID: P15692
VEGF-A(Vascular Endothelial Cell Growth Factor A)
- Detection method:
Sandwich ELISA Double Antibody
This immunoassay kit allows for the in vitro quantitative determination of VEGF-A concentrations in serum plasma and other biological fluids.
4’C for 6 months
Matrices listed below were spiked with certain level of VEGF-A and the recovery rates were calculated by comparing the measured value to the expected amount of VEGF-A in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 91-102 97 EDTA plasma(n=5) 89-100 95 UFH plasma(n=5) 90-104 95
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VEGF-A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16 serum(n=5) 90-102% 93-104% 85-97% 85-100% EDTA plasma(n=5) 84-97% 83-98% 91-100% 87-101% UFH plasma(n=5) 82-100% 80-94% 83-98% 80-98%
For Research Use Only