HYDRAGEL CSF ISOFOCUSING ELECTROPHORESIS
THE GOLD STANDARD ASSAY TO OBJECTIVELY DETECT CNS LOCAL IGG SYNTHESIS IN MULTIPLE SCLEROSIS
TEST PRESENTATION
The HYDRAGEL CSF ISOFOCUSING kit used on the HYDRASYS FOCUSING instrument provides qualitative detection and immunological characterization of IgG oligoclonal banding in human CSF and serum.
The semi-automated HYDRASYS instrument enables migration, immunofixation and direct detection on agarose gels, in order to obtain IgG profiles for qualitative analysis.
The main recommendations of European Consensus Report for Multiple Sclerosis diagnosis are:
- Isoelectrofocusing (IEF) on agarose gel for immunoglobulins separation
- Use of non-concentrated CSF material
- Simultaneous analysis of the patient’s serum and CSF
- Identical IgG concentration in paired CSF-serum samples
The presence of CSF oligoclonal bands only, characterizing local IgG synthesis, tightly correlates with the diagnosis of Multiple Sclerosis. Their presence provides useful information when in agreement with the other clinical investigations.
TEST PRINCIPLE
The HYDRAGEL CSF ISOFOCUSING assay is based on the principle of agarose gel isoelectrofocusing followed by immunofixation. The patient’s serum and CSF samples are analyzed in parallel, in order to compare the IgG distribution.
- Prior to performing the test, it is mandatory to quantify the IgGs in both liquids, in order to adjust the IgG concentration at the same level in CSF and serum.
- After the proteins separation according to their isoelectric point, the gel is directly incubated with a peroxydase labeled anti-IgG, with no need for a membrane blotting step.
- Then, the gel is processed in order to remove unbound proteins before the enzymatic reaction.
- Finally, the gel is visually evaluated.
PERFORMANCES
- Sensitivity:
- Detection limit of an IgG band: 0.031 mg/dL
- Full traceability and positive ID obtained by connecting the HYDRASYS instrument to the ASSIST pipetting station
KIT DESCRIPTION
HYDRAGEL CSF ISOFOCUSING kit: 10 gels of 3 or 9 tests
- HYDRAGEL 3 CSF ISOFOCUSING (PN 4353)
- HYDRAGEL 9 CSF ISOFOCUSING (PN 4355)
Kit content:
- Agarose gels
- Ethylene glycol solution
- Anodic and cathodic solutions
- Strips
- Sample diluent
- Antiserum diluent CSF ISOFOCUSING
- Wash solution CSF ISOFOCUSING
- Rehydrating solution
- TTF1 / TTF2 solvent
- TTF1 and TTF2 reagents
- Applicators
- Buffer containers
- Antiserum segments
- Filter papers (thin and thick)
- Plastic masks
Related product and accessories not provided in the kit:
- Quality control: CSF control (PN 4794)
- Accessory kit for HYDRASYS CSF ISOFOCUSING (PN 1258)
- Anti-IgG PER antiserum (PN 4743)
- Destaining solution (PN 4540)
- HYDRASYS wash solution (PN 4541)
COMPATIBLE INSTRUMENTS
HYDRASYS FOCUSING
HYDRASYS 2 FOCUSING
HYDRASYS 2 SCAN FOCUSING
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