LEGEND MAX™ Human CCL20 ELISA Kit with Pre-coated Plates 1 Pre-coated Plate

CCL20 (MIP-3α) belongs to the CC chemokine family. Human CCL20 is a protein of 96 amino acids, which shares the highest homology of 28% amino acid identity with MIP-1β and 64% amino acid sequence identity with the mouse CCL20. CCL20 is produced by activated monocytes, T cells, neutrophils, endothelial cells, epithelial cells and fibroblasts. Its expression is induced by lipopolysaccharide (LPS) and inflammatory cytokines such as tumor necrosis factor, IL-1β and interferon-γ, and downregulated by IL-10.

CCL20 is the ligand for chemokine receptor CCR6, which is expressed on memory T cells, B cells, and immature dendritic cells (DCs). Therefore, it is involved in the regulation of DC trafficking and recruitment and activation of T cells. CCL20 also has potent antimicrobial activity.

Upregulation of CCL20 plays important roles in autoimmune pathogenesis of the central nerve system (MS), pathogenesis of encephalomyelitis, psoriasis, rheumatoid arthritis, and inflammatory bowel diseases. CCL20 is also involved in various cancers, such as gastrointestinal cancers, pancreatic cancer, hepatocellular carcinoma, and breast cancer.

The LEGEND MAX™ Human CCL20 (MIP-3α) ELISA kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is precoated with a mouse monoclonal anti-human CCL20 antibody. The Detection Antibody is a biotinylated mouse monoclonal anti-human CCL20 antibody. This kit is specifically designed for the accurate quantitation of human CCL20 (MIP-3α) from cell culture supernatant, serum, plasma (EDTA), and other biological fluids. This kit is analytically validated with ready-to-use reagents.