LEGEND MAX™ Mouse GM-CSF ELISA Kit with Pre-coated Plates 1 Pre-coated Plate
GM-CSF (Granulocyte/Macrophage-colony stimulating factor, CSF-α, Pluripoietin-α, Eosinophil colony stimulating factor, Eo-CSF, Burst promoting activity, BPA) is a hematopoietic factor that is produced by activated T cells, B cells, mast cells, macrophages, fibroblasts and endothelial cells. In addition to supporting colony formation of granulocyte and macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte and eosinophil progenitors.
BioLegend’s LEGEND MAX™ Mouse GM-CSF ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Mouse GM-CSF specific monoclonal antibody is pre-coated on a 96-well strip-well plate.
BioLegend’s LEGEND MAX™ Mouse GM-CSF ELISA Kit is specifically designed for the accurate quantitation of Mouse GM-CSF from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.
- Reactivity
- Mouse
- Application
ELISA
- Application References
(PubMed link indicates BioLegend citation)
- Urdinguio RG,et al. 2013. Cancer Res. 79:395. PubMed.
- Urdinguio R, et al. 2013. Cancer Res. 73:395. PubMed
- Sensitivity
- 3.5 pg/mL
- Standard Range
- 15.6-1,000 pg/mL
- Materials Not Included
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Plate Shaker
- Polypropylene vials
- Kit Contents
- Anti-Mouse GM-CSF Pre-coated 96-well Strip Microplate
- Mouse GM-CSF Dectection Antibody
- Mouse GM-CSF Standard
- Matrix A (for serum and plasma samples only)
- Avidin-HRP A
- Assay Buffer A
- Wash Buffer (20X)
- Substrate Solution F
- Stop Solution
- Plate Sealers
- Cell Sources
- T cells, monocytes/macrophages, fibroblasts, endothelial cells, mast cells
- Gene ID
- NA
- UniProt
- View information about GM-CSF on UniProt.org
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