LEGEND MAX™ Mouse IL-35 Heterodimer ELISA Kit with Pre-coated Plates 1 Pre-coated Plate
IL-35 is a member of the IL-12 cytokine family. It is a heterodimeric protein composed of EBI-3 (Epstein-Barr virus-induced gene 3) and p35 (IL-12A) subunits. EBI-3 (IL-27B) is a 34 kD glycoprotein that is related to the p40 (IL-12B) subunit of IL-12. p35 is a 35 kD glycoprotein that is related to the p19 (IL-23A) and p28 (IL-27A) subunits of IL-23 and IL-27, respectively.
IL-35 is a new anti-inflammatory cytokine with many reported biological functions. It expands CD4+CD25+ Treg cells, suppresses the proliferation of CD4+CD25- effector cells, and inhibits Th17 cell polarization.
IL-35 also suppresses collagen-induced arthritis or Helicobacter-induced colitis in animal models. High levels of IL-35 were found in the plasma samples of acute myeloid leukemia patients, and synovial fluid samples of patients with rheumatoid arthritis. Relative levels of IL-35 in biological samples and its significance are not fully understood at the time of this kit release.
LEGEND MAX™ Mouse IL-35 Heterodimer ELISA kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a rat monoclonal anti-mouse IL-35 antibody. The detection antibody is a biotinylated goat polyclonal anti-mouse IL-12 antibody. This kit is specifically designed for the accurate quantitation of mouse IL-35 heterodimer from cell culture supernatant, serum, plasma, and other biological fluids. This kit is analytically validated with ready-to-use reagents.
- Reactivity
- Mouse
- Application
ELISA
- Application References
(PubMed link indicates BioLegend citation)
- Nakano S, et al. 2015. Rheumatology. 54: 1498-1506. PubMed
- Sensitivity
- 0.08 ± 0.04 ng/mL
- Standard Range
- 0.47-30 ng/mL
- Materials Not Included
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Plate Shaker
- Polypropylene vials
- Kit Contents
- Anti-Mouse IL-35 Pre-coated 96-well Strip Microplate
- Mouse IL-35 Dectection Antibody
- Mouse IL-35 Standard
- Avidin-HRP D
- Assay Buffer F
- Wash Buffer (20X)
- Substrate Solution F
- Stop Solution
- Plate Sealers
- Gene ID
- 16159View all products for this Gene ID50498View all products for this Gene ID
- UniProt
- View information about IL-35 on UniProt.org
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