LEGEND MAX™ Mouse OVA Specific IgE ELISA Kit with Pre-coated Plates 5 Pre-coated Plates
Immunoglobulin E has long been regarded as a major molecular component of atopic diseases, including allergy and asthma. Asthma is a chronic respiratory disease characterized by bronchial inflammation and airway hyperresponsiveness. Clinical studies have found a close association between asthma and serum IgE levels. Allergen-specific IgE has been detected in nasal mucosa from allergic patient. Examination of murine models of human allergic asthma has provided novel insight into the pathobiologic events linking airway inflammation with airway dysfunction.
Ovalbumin (OVA) is the main protein found in egg white, making up 60-65% of the total protein. Ovalbumin is an important protein in several different areas of research and commonly used to stimulate an allergic reaction in mice (e.g. established model allergen for airway hyperresponsiveness).
The BioLegend LEGEND MAX™ Mouse OVA specific IgE ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with OVA. This kit is specifically designed for the accurate quantitation of mouse OVA specific IgE from cell culture supernatant, serum, plasma, and other biological fluids. It is analytically validated with ready-to-use reagents.
- Reactivity
- Mouse
- Application
ELISA
- Application References
(PubMed link indicates BioLegend citation)
- Miller M, et al. 2014. J. Immunol. 192:3475. PubMed
- Nau F, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 308:191. PubMed
- Miller M, et al. 2014. J Immunol. 192:3475. PubMed
- Nau F, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 308:191. PubMed
- Ren J, et al. 2015. PLoS One. 10: e0140018. PubMed
- Fakih D, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 309: L1333 – L1343. PubMed
- Cho S, et al. 2016. J Exp Med. 213: 235 – 249. PubMed
- Li C, et al. 2016. PLoS One. 11: 0161164. PubMed
- Le H, et al. 2017. Biochem Biophys Res Commun. 10.1016/j.bbrc.2017.06.038. PubMed
- Lee H, et al. 2017. Exp Lung Res. 10.1080/01902148.2017.1339141. PubMed
- Sensitivity
- 20.7 pg/mL
- Standard Range
- 0.156-10 ng/mL
- Materials Not Included
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Plate Shaker
- Polypropylene vials
- Kit Contents
- OVA Pre-coated 96-well Strip Microplate
- Mouse IgE Dectection Antibody
- Mouse OVA specific IgE Standard
- Matrix A (for serum and plasma samples only)
- Avidin-HRP D
- Assay Buffer A
- Wash Buffer (20X)
- Substrate Solution F
- Stop Solution
- Plate Sealers
- Gene ID
- 380792View all products for this Gene ID
- UniProt
- View information about OVA-IgE on UniProt.org
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