LymphoONE™ T-Cell Expansion Xeno-Free Medium 10 x 1L
LymphoONE T-Cell Expansion Xeno-Free Medium is specifically formulated for expansion and transduction of human T lymphocytes. The medium has been optimized to enable efficient T-cell proliferation with or without the addition of human serum or plasma. LymphoONE medium contains no proteins other than pharmaceutical-grade human serum albumin and recombinant human insulin, and provides superior performance relative to other commonly used serum-free media. T-cell activation using LymphoONE T-Cell Expansion Xeno-Free Medium in combination with anti-CD3 antibody, IL-2, and RetroNectin reagent results in enhanced T-cell expansion and yields higher proportions of naïve T cells relative to approaches employing IL-2 in combination with anti-CD3 and anti-CD28 antibodies. LymphoONE medium also contains L-glutamine and streptomycin, and is confirmed to have low endotoxin levels (<0.3 EU/ml).Catalog # WK552 consists of 10 1-liter bottles of the medium.Overview Xeno-free medium that enables efficient T-cell expansion with or without the addition of serum or plasma Supplemented with human serum albumin, human insulin, L-glutamine, and streptomycin Endotoxin tested (endotoxin <0.3 EU/ml) Compatible with CultiLife culture bags for large-scale expansion of T cells Anti-CD3/IL-2 T-cell activation in the presence of RetroNectin reagent* enhances proliferation and leads to a higher proportion of naïve T cells *T-cell expansion by RetroNectin stimulation for uses other than research requires a license from Takara Bio Inc. Contact firstname.lastname@example.org for details.More Information Applications Expansion of T cells Gene transfer by lentiviral or retroviral transduction Storage Store at 4°C.Reference Janetzki, S. et al. Performance of serum-supplemented and serum-free media in IFNgamma Elispot Assays for human T cells. Cancer Immunol. Immunother. 59, 609–18 (2010).Medvec, A. R. et al. Improved expansion and in vivo function of patient T cells by a serum-free medium. Mol. Ther. Methods Clin. Dev. 8, 65–74 (2018).
Size 10 x 1L