MINICAP CDT – SEBIA – Blood Sciences – Medical Supplies Ireland
MINICAP CDT A SPECIFIC BIOMARKER FOR CHRONIC ALCOHOL ABUSE BY AUTOMATED CAPILLARY ELECTROPHORESIS FOR SMALL TO MEDIUM SIZE LABORATORIES
TEST PRESENTATION
Quantification of Carbohydrate Deficient Transferrin (CDT) by electrophoresis is used for screening patient serum samples to detect chronic alcohol abuse. During analysis, serum transferrin isoforms are separated into five major fractions according to their sialylation level:
- Asialotransferrin (non-sialylated)
- Disialotransferrin
- Trisialotransferrin
- Tetrasialotransferrin
- Pentasialotranferrin
CDT percent value is automatically calculated using the sum of low-sialylated isoforms, i.e., disialotransferrin associated with asialotransferrin (if present).
CDT levels equal to or less than 1.3% are considered normal, but CDT levels over 1.6% are abnormal and indicative of chronic alcohol abuse. CDT values over 1.3% and below 1.6% are inconclusive results.
The MINICAP CDT kit allows automatic quantification of CDT with visualization of all transferrin isoforms. Curve abnormalities can thus be detected which prevent false result reporting (such as in cirrhotic pattern).
TEST PRINCIPLE
The MINICAP CDT assay is based on the principle of capillary electrophoresis in free solution. Transferrin isoforms are separated in silica capillaries by their electrophoretic mobility and electroosmotic flow at high voltage in an alkaline buffer. Transferrin isoforms are directly detected during migration by UV absorbance.
All analysis steps are performed automatically. The sample dilution is done using a specific diluent allowing iron saturation.
PERFORMANCES
- Throughput: 10 tests/h
- Full traceability and positive ID for samples and reagents
- Rapid validation through color-coded profiles using the mosaic screen
KIT DESCRIPTION
MINICAP CDT (PN 2208) kit content:
- Buffer
- Sample diluent
- Wash solution
- CDT Wash solution
- Reagent cups
- Filters
- Bins for used cups
Related products and accessories not provided in the kit:
- Quality controls:
- Normal CDT Control (PN 4795)
- High CDT Control (PN 4772)
- CAPICLEAN (PN 2058) – only for MINICAP instrument
- CAPICLEAN MINICAP (PN 2251) – only for MINICAP FLEX-PIERCING instrument
- CAPILLARYS / MINICAP CDT samples treatment solution (PN 2054)
- CAPIprotect (2 x 5L) (PN 2061)
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