Mouse ALCAM / CD166 ELISA Kit
The ELISA Genie Mouse ALCAM / CD166 ELISA Kit can assay for Mouse ALCAM / CD166 in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.
How our Mouse ALCAM / CD166 ELISA Kits Work?
The ELISA Genie (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today’s scientists demand high quality consistent data for high impact journals, ELISA Genie have developed our range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands. Our assay kits use a quantitative sandwich ELISA technique and each kit comes with highly specific antibodies pre-coated onto a 96-well microtiter plate.
At ELISA Genie we understand the need for speed! Therefore, we have developed an ultra-fast protocol meaning you achieve your results rapidly. So, once you have prepared and plated your samples, blanks and standards, you simply incubate with a highly specific biotin-conjugated primary antibody and Avidin conjugated to Horseradish Peroxidase (HRP) and incubate for the appropriate length of time. After washing the plate according to the protocol and addition of the TMB (3,3′,5,5′-Tetramethylbenzidine) solution, the appearance of a blue colour should be detected due to an enzymatic reaction catalysed by HRP. Next step is the addition of the Stop Solution which terminates the HRP reaction and the blue colour turns yellow with the signal intensity measured on a plate reader at 450nm. The amount of bound Mouse ALCAM / CD166 is proportional to the signal generated by the reaction meaning the kit assay gives you a quantitative measurement of the analyte in your samples.
Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts. Promotes T-cell activation and proliferation via its interactions with CD6. Contributes to the formation and maturation of the immunological synapse via its interactions with CD6. Mediates homotypic interactions with cells that express ALCAM (PubMed:24740813). Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction. Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions (PubMed:23169771). Required for normal organization of the lymph vessel network (PubMed:23169771). Required for normal hematopoietic stem cell engraftment in the bone marrow (PubMed:24740813). Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow (PubMed:25730656). Promotes in vitro osteoblast proliferation and differentiation (PubMed:25730656). Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM. Mediates outgrowth and pathfinding for retinal ganglion cell axons (PubMed:15345243).
- Post-Translational Modification:
- Uniprot ID: Q61490
- Detection method:
Sandwich ELISA Double Antibody
This immunoassay kit allows for the in vitro quantitative determination of ALCAM/CD166/MEMD/activated leucocyte cell adhesion molecule/CD166 antigen concentrations in serum plasma and other biological fluids.
4’C for 6 months
Matrices listed below were spiked with certain level of ALCAM/CD166/MEMD/activated leucocyte cell adhesion molecule/CD166 antigen and the recovery rates were calculated by comparing the measured value to the expected amount of ALCAM/CD166/MEMD/activated leucocyte cell adhesion molecule/CD166 antigen in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 88-105 98 EDTA plasma(n=5) 87-99 90 UFH plasma(n=5) 89-103 95
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ALCAM/CD166/MEMD/activated leucocyte cell adhesion molecule/CD166 antigen and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16 serum(n=5) 89-103% 87-98% 88-100% 88-103% EDTA plasma(n=5) 91-98% 85-95% 83-97% 88-97% UFH plasma(n=5) 80-85% 81-99% 83-97% 80-99%
For Research Use Only