Mouse Alpha MSH / Alpha-Melanocyte Stimulating Hormone ELISA Kit
The ELISA Genie Mouse Alpha MSH / Alpha-Melanocyte Stimulating Hormone ELISA Kit can assay for Mouse Alpha MSH / Alpha-Melanocyte Stimulating Hormone in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.
How our Mouse Alpha MSH / Alpha-Melanocyte Stimulating Hormone ELISA Kits Work?
The ELISA Genie (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today’s scientists demand high quality consistent data for high impact journals, ELISA Genie have developed our range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands. Our assay kits use a quantitative sandwich ELISA technique and each kit comes with highly specific antibodies pre-coated onto a 96-well microtiter plate.
At ELISA Genie we understand the need for speed! Therefore, we have developed an ultra-fast protocol meaning you achieve your results rapidly. So, once you have prepared and plated your samples, blanks and standards, you simply incubate with a highly specific biotin-conjugated primary antibody and Avidin conjugated to Horseradish Peroxidase (HRP) and incubate for the appropriate length of time. After washing the plate according to the protocol and addition of the TMB (3,3′,5,5′-Tetramethylbenzidine) solution, the appearance of a blue colour should be detected due to an enzymatic reaction catalysed by HRP. Next step is the addition of the Stop Solution which terminates the HRP reaction and the blue colour turns yellow with the signal intensity measured on a plate reader at 450nm. The amount of bound Mouse Alpha MSH / Alpha-Melanocyte Stimulating Hormone is proportional to the signal generated by the reaction meaning the kit assay gives you a quantitative measurement of the analyte in your samples.
- Description:
Zinc metalloprotease that specifically cleaves ‘Lys-63’-linked polyubiquitin chains. Does not cleave ‘Lys-48’-linked polyubiquitin chains. Plays a role in signal transduction for cell growth and MYC induction mediated by IL-2 and GM-CSF. Potentiates BMP (bone morphogenetic protein) signaling by antagonizing the inhibitory action of SMAD6 and SMAD7. Involved in the ubiquitin-dependent sorting and trafficking of receptors from endosomes to lysosome. Endosomal localization of STAMBP is required for efficient EGFR degradation but not for its internalization. Involved in the negative regulation of PI3K-AKT-mTOR and RAS-MAP signaling pathways.
- Post-Translational Modification:
Phosphorylated after BMP type I receptor activation. Ubiquitinated by SMURF2 in the presence of RNF11.
- Uniprot ID: Q9CQ26
- Alias:
alpha MSH
- Detection method:
Sandwich ELISA Double Antibody
- Application:
This immunoassay kit allows for the in vitro quantitative determination of alpha MSH concentrations in serum plasma and other biological fluids.
- Size:
96T
- Range:
15.625-1000pg/ml
- Sensitivity:
< 9.375pg/ml
- Storage:
4’C for 6 months
- Recovery:
Matrices listed below were spiked with certain level of alpha MSH and the recovery rates were calculated by comparing the measured value to the expected amount of alpha MSH in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 86-103 97 EDTA plasma(n=5) 89-105 97 UFH plasma(n=5) 89-104 96 - Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of alpha MSH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16 serum(n=5) 94-105% 85-102% 85-98% 87-105% EDTA plasma(n=5) 83-101% 86-95% 83-99% 85-100% UFH plasma(n=5) 84-98% 82-92% 81-100% 81-100% - CV(%):
Intra-Assay: CV<8%
Inter-Assay: CV<10% - Note:
For Research Use Only
Reactivity | Mouse |
---|---|
ELISA Type | Sandwich |
Reviews
There are no reviews yet.