Mouse Pro-opiomelanocortin (Pomc) ELISA Kit
The ELISA Genie Mouse Pro-opiomelanocortin (Pomc) ELISA Kit can assay for Mouse Pro-opiomelanocortin in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.
How do our Mouse Pro-opiomelanocortin (Pomc) ELISA Kits work?
The ELISA Genie Mouse Pro-opiomelanocortin (Pomc) ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Pro-opiomelanocortin. During the reaction, in the sample or standard competes with a fixed amount of biotin-labeled for sites on a pre-coated Monoclonal antibody specific to Mouse Pro-opiomelanocortin. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Gene name
Pomc
Protein Names/Synonym
Pro-opiomelanocortin, POMC, Corticotropin-lipotropin, Pomc, Pomc1
Reactivity | Mouse |
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ELISA Type | Sandwich |
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