NA/NE/Noradrenaline/Norepinephrine ELISA Kit
The NA / NE / Noradrenaline / Norepinephrine ELISA Kit can assay for NA / NE / Noradrenaline / Norepinephrine in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.
How our NA / NE / Noradrenaline / Norepinephrine ELISA Kits Work?
The ELISA Genie ELISA (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today’s scientists demand high quality consistent data for high impact journals, We have developed a range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands.
This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with NA / NE / Noradrenaline / Norepinephrine. During the reaction, NA / NE / Noradrenaline / Norepinephrine in the sample or standard competes with a fixed amount of NA / NE / Noradrenaline / Norepinephrine on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to NA / NE / Noradrenaline / Norepinephrine. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NA / NE / Noradrenaline / Norepinephrine in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Product name:
NA/NE(Noradrenaline/Norepinephrine) ELISA Kit
- Catalogue No.:
- Detection method:
Competitive ELISA Coated with Antigen
This immunoassay kit allows for the in vitro quantitative determination of NA/NE concentrations in serum plasma and other biological fluids.
4 „ƒ for 6 months
Matrices listed below were spiked with certain level of NA/NE and the recovery rates were calculated by comparing the measured value to the expected amount of NA/NE in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 92-101 96 EDTA plasma(n=5) 85-102 96 UFH plasma(n=5) 88-100 95
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NA/NE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16 serum(n=5) 97-103% 88-105% 85-98% 86-103% EDTA plasma(n=5) 82-98% 82-94% 85-93% 84-90% UFH plasma(n=5) 85-96% 94-100% 89-100% 81-97%
For Research Use Only