PrimerArray® Cytokine-Cytokine Receptor Interaction (Human)
Takara Bio’s PrimerArray Series offers primer sets for real-time RT-PCR (qPCR) that can be used for gene expression analysis of biological pathways. Each PrimerArray Series primer set contains 96 total primers that allow for the simultaneous analysis of 88 biological pathway-related genes and 8 housekeeping genes. The performance of each primer set for real-time RT-PCR (qPCR) has been validated.
Affordability: Each primer set for real-time RT-PCR (qPCR) supplies sufficient primer for up to 12 qPCR experiments (using a 25 µl qPCR reaction volume)
Minimizes pipetting steps and reduces error
Enables high-throughput gene expression analysis of biological pathways
Optimized for superior performance with SYBR Ex Taq qPCR kits
PrimerArray Analysis Tool makes data analysis easy and allows data visualization on pathway maps
Compatible with all standard real-time PCR instruments
Size: 12 x 96 Rxns
Gene expression analysis of biological pathways including cytokine-cytokine receptor interactions, cell cycling, cell adhesion, Jak-STAT signaling, natural killer cell mediated cytotoxicity, axon guidance, focal adhesion, T cell receptor signaling, TGF-beta signaling, Wnt signaling.
Procedural overview for gene expression analysis by real-time RT-PCR
The overall workflow for the PrimerArray Series primer sets is straightforward:
RNA extraction (30 min): NucleoSpin RNA (Cat. # 740955.50) or RNAiso (Cat. # 9108, not available in all geographic locations) is recommended.
Reverse transcription (20 min): PrimeScript RT Master Mix (Perfect Real Time) (Cat. # RR036A) is recommended.
Dispense real-time PCR reaction solution (10 min): Prepare a master mix containing the cDNA sample and SYBR Premix Ex Taq II (Tli RNase H Plus) (Cat.# RR820A); dispense into a 96-well plate.
Add PrimerArray primers (5 min): Use 4 µl of PrimerArray Series primers per reaction
Real-time PCR (1–2 hours): PrimerArray Series primers are compatible with all commonly used real-time PCR systems
Data analysis (30 minutes): Use the PrimerArray Analysis Tool
Hirano, S., Fujitani, Y., Furuyama, A. & Kanno, S. Uptake and cytotoxic effects of multi-walled carbon nanotubes in human bronchial epithelial cells. Toxicol. Appl. Pharmacol.249, 8–15 (2010).
Yoshioka, R. et al. Corneal epithelial wound healing impaired in keratinocyte-specific HB-EGF-deficient mice in vivo and in vitro. Invest. Ophthalmol. Vis. Sci.51, 5630–9 (2010).