Rat IFN-alpha ELISA Kit
- Product name:
Rat IFN-α (Interferon α) ELISA Kit
- Catalogue No.:
RTFI00888
- Detection method:
Sandwich ELISA, Double Antibody
- Application:
IFN-α ELISA Kit allows for the in vitro quantitative determination of IFN-α concentrations in plasma, tissue homogenates and other biological fluids.
- Size:
96T
- Range:
15.625-1000pg/ml
- Sensitivity:
< 9.375pg/ml
- Storage:
4 ℃ for 6 months
- Recovery:
Matrices listed below were spiked with certain level of IFN-α and the recovery rates were calculated by comparing the measured value to the expected amount of IFN-α in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 86-103 91 EDTA plasma(n=5) 85-105 95 heparin plasma(n=5) 85-102 94 - Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFN-α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16 serum(n=5) 88-103% 85-103% 90-102% 90-105% EDTA plasma(n=5) 82-94% 86-101% 82-98% 90-101% heparin plasma(n=5) 85-100% 84-100% 89-99% 80-96% - CV(%):
Intra-Assay: CV<8%
Inter-Assay: CV<10% - Note:
For Research Use Only
ELISA Type | Sandwich |
---|---|
Reactivity | Rat |
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