These premade, highly concentrated and purified lentiviral particles deliver sequences that express fluorescent protein tags (AcGFP1 or mCherry) targeted to specific subcellular locations. Particles are available for visualization of the nucleus, endoplasmic reticulum, mitochondria, endosomes, golgi, cytoskeleton, and plasma membrane
- What does 109 TU/ml mean?
- In each microliter, there will be at least 1 x 106 functional lentivirus particles.
- Each 60-µl kit is sufficient for 30 transductions (using an MOI of 10) in a 6-well plate containing 2 x 105 cells/well.
- Ready-to-use particles can be added directly to cells:
- Highly concentrated lentiviral particles; all lots are >109 transducing units (TU) per ml (functional titer, not a just a particle count).
- High-purity lentiviral particles—transduce cells without cytotoxicity.
- Expression can be monitored in living cells by fluorescence microscopy
- AcGFP1 is a true monomeric green fluorescent protein with spectral properties similar to those of EGFP.
- mCherry is the most widely adopted monomeric red fluorescent protein.
- Learn more about the properties of these fluorescent proteins.
- Particles are available for labeling the nucleus, endoplasmic reticulum, actin, mitochondria, tubulin, endosome, golgi, and plasma membrane.
- These vectors have a puromycin resistance cassette and the stable clones integrating these constructs can be selected using puromycin.
Size: 3 x 20 uL
- Cell organelle/structure labeling—monitor protein localization, protein trafficking, organelle dynamics, etc.
- Simple method for generating stable reporter cell lines