Hydragel hemoglobin(E) gel electrophoresis for first line hemoglobin testing
TEST PRESENTATION
Hemoglobin electrophoresis is a well-established technique routinely used in clinical laboratories to screen samples for hemoglobinopathies(hemoglobin variants and thalassemias).
The HYDRAGEL HEMOGLOBIN(E) kit enables, on HYDRASYS instruments, the separation of the normal hemoglobins (A and A2) and the detection of the major hemoglobin variants: S or D and C or E, by electrophoresis on alkaline agarose gels.
The semi-automated HYDRASYS instrument offers sample application, electrophoretic migration and staining.
The visualization of the gel provides qualitative analysis, while the reading of the agarose gels on a Sebia reader provides hemoglobin profiles for relative quantitative analysis.
On the HYDRASYS 2 SCAN, the scanning system is embedded in the instrument.
Electrophoresis on acidic gel, like HYDRAGEL ACID(E) HEMOGLOBIN(E), should follow to confirm the identification of hemoglobin variants, in particular, to differentiate hemoglobins S from D and E from C.
TEST PRINCIPLE
The HYDRAGEL HEMOGLOBIN(E) assay is based on the principle of electrophoresis separation on agarose gel at alkaline pH (pH 8.5).
Prior to performing the test, it is mandatory to prepare the samples (hemolyzed washed red blood cells).
The HYDRASYS system is a semi-automated multi-parameter instrument. The automated steps include processing of the agarose gel in the following sequence:
- Sample application
- Electrophoretic migration
- Drying
- Staining with amidoblack dye
- Destaining
- Final drying
PERFORMANCES
- Resolution:
- Optimal focalization of the fractions obtained by applying a very thin sample’s application using the Sebia proprietary applicators.
KIT DESCRIPTION
HYDRAGEL HEMOGLOBIN(E) kit: 10 gels of 7 or 15 tests
- HYDRAGEL 7 HEMOGLOBIN(E) (PN 4106)
- HYDRAGEL 15 HEMOGLOBIN(E) (PN 4126)
Kit content:
- Agarose gels
- Buffered strips
- Ethylene glycol solution
- Hemolyzing solution
- Amidoblack stain
- Staining solution diluent
- Applicators
- Filter papers
Related product and accessories not included in the kit:
- Quality controls:
- Normal Hb A2 Control (PN 4778)
- Pathological Hb A2 Control (PN 4779)
- Hb AFSC Control (PN 4792)
- Destaining Solution (PN 4540)
- HYDRASYS Wash Solution (PN 4541)
COMPATIBLE INSTRUMENTS
HYDRASYS
HYDRASYS FOCUSING
HYDRASYS 2
HYDRASYS 2 SCAN
HYDRASYS 2 FOCUSING
HYDRASYS 2 SCAN FOCUSING
