ST/5-HT/Serotonin/5-Hydroxytryptamine ELISA Kit
The Serotonin ELISA Kit can assay for ST / 5-HT / Serotonin / 5-Hydroxytryptamine in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues.
How our ST / 5-HT / Serotonin / 5-Hydroxytryptamine ELISA Kits Work?
The ELISA Genie ELISA (enzyme-linked immunosorbent assays) assay kits are designed for the quantitative measurement of analytes in a wide variety of samples. As today’s scientists demand high quality consistent data for high impact journals, We have developed a range of sensitive, fast and reliable ELISA kit assays to meet and exceed those demands.
This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with ST / 5-HT / Serotonin / 5-Hydroxytryptamine. During the reaction, ST / 5-HT / Serotonin / 5-Hydroxytryptamine in the sample or standard competes with a fixed amount of ST / 5-HT / Serotonin / 5-Hydroxytryptamine on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to ST / 5-HT / Serotonin / 5-Hydroxytryptamine. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ST / 5-HT / Serotonin / 5-Hydroxytryptamine in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Product name:
ST/5-HT(5-hydroxytryptamine) ELISA Kit
- Catalogue No.:
- Detection method:
Competitive ELISA Coated with Antigen
This immunoassay kit allows for the in vitro quantitative determination of ST/5-HT concentrations in serum plasma and other biological fluids.
4 „ƒ for 6 months
Matrices listed below were spiked with certain level of ST/5-HT and the recovery rates were calculated by comparing the measured value to the expected amount of ST/5-HT in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 92-103 97 EDTA plasma(n=5) 92-102 97 UFH plasma(n=5) 87-103 93
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ST/5-HT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16 serum(n=5) 92-104% 85-96% 85-105% 87-100% EDTA plasma(n=5) 84-93% 83-98% 83-100% 84-99% UFH plasma(n=5) 81-96% 89-95% 81-98% 80-95%
For Research Use Only