SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing

The SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing is the fourth generation of SMARTer Ultra Low products and generates high-quality cDNA from ultra-low amounts of total RNA, or directly from 1–1,000 intact cells. This kit can accommodate an input volume of 10 μl and is regularly tested with 10 pg of total RNA. It also performs robust cDNA synthesis from a single cell (equivalent to 10 pg of total RNA), generating data with the highest sensitivity compared to previous generations. This kit incorporates locked nucleic acid (LNA) technology and improves on the SMART-Seq2 method. cDNA libraries generated by this kit have been tested for compatibility with Ion Torrent and Illumina sequencing platforms. SMART technology offers unparalleled sensitivity and unbiased amplification of cDNA transcripts. Most importantly, SMART technology enriches for full-length transcripts and maintains the true representation of mRNA transcripts from the original sample. These factors are critical for transcriptome sequencing and gene expression analysis.