Tet-On® 3G Bidirectional Inducible Expression System (EF1alpha, ZsGreen1)
EF1-alpha promoter versions of the Tet-On 3G inducible expression system provide for consistent long-term expression of the Tet-On 3G transactivator, even in cell types known for their tendency to silence a CMV promoter over time, such as hematopoietic cells and stem cells. This means that in these cell types, long-term tetracycline-inducible expression can be achieved.
- Tet-On 3G tetracyline transactivator is expressed from an EF1-alpha promoter
- Very low background expression
- High induced expression
- Inducible expression in cell types known to silence CMV promoters, such as hematopoietic cells and stem cells