LEGEND MAX™ Human IL-21 Kit with Pre-coated Plates 5 Pre-coated Plates
Human IL-21 is a 4-helix-bundle-type fold cytokine with significant homology to IL-2, IL-4, and IL-15. IL-21 is produced most abundantly by activated CD4+ T cells, including Th17 lineage cells and natural killer T (NKT) cells, and has pleiotropic effects on both innate and adaptive immune responses. These actions include positive effects such as enhanced proliferation of NK cells and cytotoxic T cells that can destroy virally infected or cancerous cells and direct inhibitory effects on the antigen-presenting function of dendritic cells. It can also be proapoptotic for B cells and NK cells.
BioLegend’s LEGEND MAX™ Human IL-21 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a human IL-21 specific monoclonal antibody is pre-coated on a 96-well strip-well plate.
This Human IL-21 ELISA Kit is specifically designed for the accurate quantitation of human IL-21 from cell culture supernatant, serum, plasma, and other bodily fluids. It is ready-to-use, accurate, and sensitive.
- Reactivity
- Human
- Application
ELISA
- Application References
(PubMed link indicates BioLegend citation)
- Isaza-Guzman DM, et al. 2015. Arch Oral Biol. 60:91. PubMed
- Isaza-Guzmán D, et al. 2015. Arch Oral Biol. 60:91. PubMed
- Wang T, et al. 2016. Vascular Med. 21: 99-104. PubMed
- Sensitivity
- 4.2 pg/mL
- Standard Range
- 31.3-2,000 pg/mL
- Materials Not Included
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Plate Shaker
- Polypropylene vials
- Kit Contents
- Anti-Human IL-21 Pre-coated 96-well Strip Microplate
- Human IL-21 Dectection Antibody
- Human IL-21 Standard
- Avidin-HRP D
- Assay Buffer A
- Wash Buffer (20X)
- Substrate Solution F
- Stop Solution
- Plate Sealers
- Cell Sources
- NK-T cells and T cells
- Gene ID
- NA
- UniProt
- View information about IL-21 on UniProt.org
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