Description
qPCR From RNA
qPCRBIO Probe 1-Step Go is a universal probe kit designed for fast, highly specific and sensitive probe-based real-time RT-PCR. We use the latest developments in reverse transcriptase technology and buffer chemistry to give efficient cDNA synthesis and real-time PCR in a single tube.
qPCRBIO Probe 1-Step Go is engineered for use on a wide range of probe technologies including TaqMan®, Scorpions® and molecular beacons probes. The kit includes a 2x mix and can be used to quantify any RNA template including mRNA, total RNA and viral RNA sequences. qPCRBIO Probe 1-Step Go is designed to give rapid and accurate results over a broad range of template concentrations and is ideally suited to the detection of RNA viruses including SARS-CoV-2.
The kit includes a thermostable and extremely active modified MMLV reverse transcriptase (RTase Go) and advanced RNase inhibitor that prevents degradation of RNA by contaminating RNase. Antibody-mediated hot start technology prevents the formation of primer dimers and non-specific products giving highly specific and ultra-sensitive real-time RT-PCR with unrivalled efficiency in multiplex. Combining the latest developments in polymerase technology and advanced buffer chemistry we offer market-leading performance with minimal or no optimisation.
Features
- Rapid and sensitive detection of RNA viruses including SARS-CoV-2
- Rapid extension rate for early Ct values
- Market-leading sensitivity – increased limit of detection
- High efficiency in multiplex reactions
- Advanced RNase inhibitor
- Antibody-mediated hot start PCR
- Compatible with all real-time PCR platforms – standard and fast cycling conditions
Applications
- COVID-19 / SARS-CoV-2 testing and research
- Diagnostic real-time PCR
- Multiplex or singleplex
- Detection of low copy number targets
- Absolute quantification
- Relative gene expression analysis
- TaqMan®, Scorpions® and molecular beacon probes
Size
300 x 20 µL rxns
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