Polypropylene wells, polycarbonate frame, cut corner A24, working volume: <30 µl, total well capacity: 55 µl; designed for use on standard thermal cyclers
Our FrameStar® 384 Well Skirted PCR Plates are designed for high-throughput PCR. They are compatible with the majority of 384 well block PCR, qPCR and sequencing instruments, and the rigid 2-component design eliminates warping and distortion during PCR making it ideal for use with robotic systems.
The skirt allows for labelling or barcoding.
While clear wells are the best for sample visibility and optical appeal, white wells increase the signal-to-noise ratio by maximising reflect of light in fluorescent based assays used in real-time PCR and qPCR. This leads to a better sensitivity and reproducibility from a wide range of qPCR instruments from most manufacturers.
However, white well PCR plates may lead to software related problems with ABI®/Life Technologies® cyclers. For this reason the manufacturer recommends the use of plates with frosted wells as an alternative to white wells. Although the frosting does not provide the same sensitivity as white wells, readings are still considerably higher than those achieved with standard clear wells.
In response to this, we at 4titude® have developed a frosted well variety of the FrameStar® 384 well plate to offer ABI®/Life Technologies® instrument users the time and money-saving advantages of FrameStar®. And as the signal levels are directly comparable with ABI®/Life Technologies® plates, there’s no need for users to recalibrate their instruments.
Find these plates in the Order tab under product code 4ti-0387.
Frosted wells compared to clear wells
This plate is available with linear Code 128-format barcodes to aid identification and traceability for your samples. To order your plates with barcodes, simply add /SBC to the end of the ordering code for Standard Single Barcoding, /DBC for Standard Double Barcoding, /TBC for Standard Triple Barcoding, or /QBC for Standard Quadruple Barcoding (see Barcoding for more details).
| Plate Width | 127.76 ± 0.25 mm |
| Plate Depth | 85.48 ± 0.25 mm |
| Plate Height | 10.60 ± 0.25 mm |
| Well Depth | 9.60 ± 0.10 mm |
| Well Diameter | 3.10 ± 0.10 mm |
| Distance to centre of A1 from top edge | 8.99 ± 0.25 mm |
| Distance to centre of A1 from left edge | 12.13 ± 0.25 mm |
| Pitch (distance between A1 and A2) | 4.50 mm |
Genomics Plastics
Genomics Plastics
