Description
Probe Based Assays – Universal – 50 x 1 mL pouch
Our universal probe kit is designed to give superior sensitivity and specificity in all probe-based real-time PCR assays, including TaqMan®, Scorpions®, and molecular beacon probes. qPCRBIO Probe Blue Mix contains a non-reactive dye to improve reaction mix visibility and is particularly useful in reducing pipetting errors when loading small volume reactions or using white plates.
By combining antibody-mediated hot start technology with the latest advances in buffer chemistry we offer market-leading performance with minimal or no optimisation required. qPCRBIO Probe Mix can be used to reliably detect extremely low copy number targets and quantify any DNA template including genomic, cDNA and viral sequences. The enhanced sensitivity of qPCRBIO Probe Blue Mix makes it the perfect choice for multiplexing.
Use our qPCR Selection Tool to find out which ROX variant is compatible with your instrument.
Features
- Easy-to-see blue mix for greater pipetting precision
- High efficiency in multiplex reactions
- Rapid extension rate for early Ct values
- Market leading sensitivity – increased limit of detection
- Efficient amplification from GC and AT-rich sequences
- Antibody-mediated hot start technology
- Compatible on all real-time PCR platforms – standard and fast cycling conditions
Applications
- Absolute quantification
- Relative gene expression analysis
- TaqMan®, Scorpions® and molecular beacon probes
- Detection of extremely low copy number targets
- Diagnostic real-time PCR
- Genotyping & allelic discrimination
Size
5000 x 20 µL rxns
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