Description
qPCR From RNA
qPCRBIO SyGreen 1-Step Kits have been designed for fast, highly specific and ultra-sensitive real-time RT-PCR. We use the latest developments in reverse transcriptase technology and buffer chemistry to give efficient cDNA synthesis and real-time PCR in a single tube.
qPCRBIO SyGreen 1-Step Kits can be used to quantify any RNA template including mRNA, total RNA and viral RNA sequences. qPCRBIO SyGreen 1-Step Detect is designed for sensitivity and is ideally suited to the detection of extremely low copy number targets. qPCRBIO SyGreen 1-Step Go gives the earliest Ct and is formulated for rapid and accurate results from high template concentrations.
The kits include a thermostable and extremely active modified MMLV reverse transcriptase and advanced RNase inhibitor that prevents degradation of RNA by contaminating RNase. PCR amplification is powered by antibody-mediated hot start technology, preventing the formation of primer dimers and non-specific products leading to improved reaction sensitivity and specificity. Combining the latest developments in polymerase technology and advanced buffer chemistry we offer market- leading performance with minimal or no optimisation.
Features
- Thermostable reverse transcriptase 45°C to 55°C
- Advanced RNase inhibitor
- Non-PCR inhibiting intercalating dye
- Rapid extension rate for early Ct values
- Market-leading sensitivity
- Increased limit of detection
- Antibody-mediated hot start PCR
- Compatible on all standard and fast cycling real-time PCR platforms
Applications
- Absolute quantification
- Relative gene expression analysis
- Detection of extremely low copy number targets
- qPCRBIO SyGreen 1-Step Detect recommended for template amounts of 1pg – 10ng total RNA or >0.01pg mRNA per reaction
- qPCRBIO SyGreen 1-Step Go recommended for template amounts of 10pg – 100ng total RNA or >0.01pg mRNA per reaction
Size
1200 x 20 µL rxns
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