Description
SyGreen real-time PCR – 50 x 1 mL pouch
Our blue mixes contain a non-reactive dye to improve reaction mix visibility, allowing greater pipetting precision and reduced errors without affecting your real-time PCR performance. qPCRBIO SyGreen Blue Mix can be used to quantify any DNA template including genomic, cDNA and viral sequences, and is able to reliably detect extremely low copy number targets. The antibody-mediated hot start system prevents the formation of primer dimers and non-specific products leading to greatly improved reaction sensitivity and specificity.
This 2x mix uses a proprietary intercalating dye that does not inhibit PCR, unlike other popular fluorescent dyes. The same developments that improve the sensitivity and consistency of qPCRBIO SyGreen Blue Mix in standard cycling conditions also allow for industry-leading performance in fast and ultra fast cycling conditions.
Use our qPCR Selection Tool to find out which ROX variant is compatible with your instrument.
Features
- Easy-to-see blue mix for greater pipetting precision and accuracy
- Rapid extension rate for early Ct values
- Market-leading sensitivity – increased limit of detection
- Specific amplification from complex templates including GC-rich sequences
- Non-PCR inhibiting intercalating dye
- Compatible with all standard and fast cycling real-time instruments
Applications
- Absolute quantification
- Relative gene expression analysis
- High-throughput qPCR from genomic, cDNA and viral sequences
- Detection of extremely low copy number targets
- Crude sample PCR
Size
5000 x 20 µL rxns
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