UltraScript 2.0 Reverse Transcriptase

Description

cDNA Synthesis

The enhanced thermostability of UltraScript 2.0 Reverse Transcriptase (RTase) allows reaction temperatures of over 55°C to be used, giving improved specificity, higher cDNA yields, and more full length cDNA product. The enzyme remains partially active even up to 90°C and is designed for efficient reverse transcription of the most difficult RNA templates, including GC-rich and highly structured transcripts. 

UltraScript 2.0 RTase is designed for efficient cDNA synthesis from a broad range of RNA concentrations. UltraScript 2.0 kits can be used with 20pg to 3.5μg total RNA or oligo(dT) purified mRNA. The RTase is blended with an advanced RNase inhibitor preventing degradation of RNA by contaminating RNase.

UltraScript 2.0 RTase is available in three convenient formats, each with dNTPs, MgCl₂, and RNase inhibitor included. Our stand-alone enzyme with 5x buffer gives users the flexibility to define their own priming strategy and offers exceptional performance with gene-specific primers, oligo(dT) and random hexamers.

The 2-tube UltraScript 2.0 cDNA Synthesis Kit contains all the components required for fast, reliable and unbiased cDNA synthesis, including an optimised blend of anchored oligo(dT) and random hexamers. UltraScript 2.0 cDNA Synthesis Kit Separate Oligos provides anchored oligo(dT) and random hexamers in separate tubes, for user optimisation depending on the type of analysis needed.

Features

• Highly thermostable reverse transcriptase 55°C to 65°C and above
• Advanced RNase inhibitor
• High cDNA yields from as little as 20pg total RNA
• Accurate reverse transcription of GC-rich and highly structured transcripts
• High sensitivity for challenging templates
• Reduced RNase H activity
• Available as a stand-alone enzyme with buffer, a cDNA synthesis kit with premixed oligos and a cDNA synthesis kit with separate oligos

Applications

• cDNA synthesis for PCR analysis, cloning, cDNA library preparation and Next Generation Sequencing
• Viral RNA targets
• miRNA targets
• Efficient synthesis from total RNA or poly(A)+ RNA

Size

40,000 units