LEGEND MAX™ Rat CCL11 ELISA Kit with Pre-coated Plates 5 Pre-coated Plates
CCL11, also known as Eotaxin, is a small cytokine belonging to the CC chemokine family. The full length of CCL11 is 97 amino acids (aa) with a 23 aa signal peptide, which is cleaved to generate a 74 aa mature protein. The molecular mass of CCL11 is about 10 kD, and its gene is located on the q arm of chromosome 17. CCL11 expression has been found in normal tissues such as smooth muscle and small intestine. Endothelial cells, smooth muscle cells, epithelial cells, alveolar macrophages, and eosinophils are known to produce CCL11. Rat CCL11 shares a 61% and 96% amino acid sequence identity to both human and mouse, respectively.
CCL11 is produced when inflammatory cytokines, such as IL-5 and TNF-α, stimulate lung endothelial cells, fibroblasts, and smooth muscle cells in response to allergens. As a potent eosinophil chemoattractant, via binding to its major receptor, CCR3, CCL11 efficiently induces the accumulation of eosinophils, which is a response associated with allergic inflammation. Therefore, atopic dermatitis, asthma, and parasitic infections are a few of the eosinophilic inflammatory diseases directly related to CCL11. In addition, CCL11 induces the formation of blood vessels and other angiogenic responses which are accompanied by inflammatory infiltration and is also involved in the regulation of tumor development.
The LEGEND MAX™ Rat CCL11 ELISA kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a rat monoclonal anti-CCL11 antibody. The detection antibody is a biotinylated goat polyclonal anti-CCL11 antibody. This kit has been specifically validated for the accurate quantitation of rat CCL11 from tissue homogenates, serum, plasma, and other biological fluids. This kit is analytically validated with ready-to-use reagents.
- Reactivity
- Rat
- Application
ELISA
- Sensitivity
- 0.825 ± 0.386 pg/mL
- Standard Range
- 6.25 – 400 pg/mL
- Materials Not Included
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Plate Shaker
- Polypropylene vials
- Kit Contents
- Anti-Rat CCL11 Pre-coated 96 well Strip Microplate
- Rat CCL11 Detection Antibody
- Rat CCL11 Standard
- Avidin-HRP
- Assay Buffer B
- Wash Buffer (20X)
- Substrate Solution F
- Stop Solution
- Plate Sealers
- Gene ID
- 29397View all products for this Gene ID
- UniProt
- View information about CCL11 on UniProt.org
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