Fecal DNA Purification Kit – 50 Prep
Bead Mill Fecal DNA Purification Mini Kit – 50 Prep
The Omni Fecal DNA Purification Kit contains silica based spin-capture columns and nontoxic reagents. The optimized reagents are designed specifically for microbial genomic DNA extraction from stool and urine. After sample lysis using the Omni pre-filled bead beating tubes, microbial DNA is separated from PCR inhibitors such as humic compounds. Microbial DNA is then purified through spin-column capture. Genomic DNA purified using the Omni Fecal DNA Kit is ready for downstream applications such as PCR, southern blots and restriction enzyme digestion.
Product Features:
- Complete DNA purification kit including spin-columns and nontoxic reagents
- Effective removal of PCR inhibitors including humic compounds
- Microbial DNA isolation from a variety of organisms including, gram-positive and gram-negative bacteria
- Isolation of pure DNA from up to 250 mg stool, biosolids, gut material and urine
- Rapid sample disruption with 0.5 mm glass beads
- Recover up to 25 ug DNA per filter
Illustrated Protocol
Ordering Information: Unit includes 50 spin-columns, 50 pre-filled bead tubes with 0.5 mm glass beads, reagents suitable for 50 purifications and instruction guide
- DNA types: Microbial genomic DNA
- DNA purity: High quality genomic DNA with A260/A280 > 1.8
- Inhibitor removal: DNA is separated from humic compounds and other PCR inhibitors prior to purification
- DNA yield: Up to 25 ug per filter
- Capacity: Capable of microbial DNA extraction from up to 250 mg of stool, gut material, water and urine
- Lysis method: Pre-filled 0.5 mm glass bead tubes on a bead mill homogenizer or vortex mixer
- Equipment required: Bead mill or vortexer, microcentrifuge, 1.5 mL microcentrifuge tubes, water bath, heat block, 100% ethanol and isopropanol
Reviews
There are no reviews yet.