Human ACE-2 ELISA

Human ACE-2 ELISA

Antigen Information

Accession Number: Q9BYF1
Gene ID: 59272
Gene Symbols: ACE2UNQ868/PRO1885
Protein Name & Synonyms: Angiotensin-converting enzyme 2 (EC:3.4.17.23) (ACE-related carboxypeptidase) (Angiotensin-converting enzyme homolog) (ACEH) (Metalloprotease MPROT15) [Cleaved into: Processed angiotensin-converting enzyme 2] Target Species: Human

Assay Format

Detection Range: 0.025 ng/ml – 20 ng/ml
Recommended Dilution (serum & plasma): 8 fold
Sensitivity: 0.025 ng/ml
Species Detected: Human
Specificity: This ELISA antibody pair detects Human ACE-2. Other species not determined.
Number of Targets Detected: 1
Species Detected:  Human
Compatible Sample Types: Cell Culture SupernatantsCell LysatesPlasmaSerumTissue Lysates
Design Principle: Sandwich-based
Method of Detection: Colorimetric
Quantitative/Semi-Quantitative: Quantitative
Solid Support: 96-well Microplate

Product Specifications

Size:1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Application Notes

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Other Materials Required

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Protocol Outline

• Prepare all reagents, samples and standards as instructed in the manual.
• Add 100 µl of standard or sample to each well.
• Incubate 2.5 h at RT or O/N at 4°C.
• Add 100 µl of prepared biotin antibody to each well.
• Incubate 1 h at RT.
• Add 100 µl of prepared Streptavidin solution to each well.
• Incubate 45 min at RT.
• Add 100 µl of TMB One-Step Substrate Reagent to each well.
• Incubate 30 min at RT.
• Add 50 µl of Stop Solution to each well.
• Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.