Human TNF-a ELISA MAX™ Deluxe 20 Plates | Medical Supply Company

Human TNF-a ELISA MAX™ Standard 5 Plates

SKU: 430201 Categories: ELISA, Human, Sandwich, Standard Set Tag: Biolegend BV

Description
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Human TNF-a ELISA MAX™ Standard 5 Plates

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend’s ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend’s ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Reactivity

Human

Application

ELISA

As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.

Application References (PubMed link indicates BioLegend citation)

Davis AM, et al. 2008. J. Immunol. 180:6923. PubMed
Kulkarni O, et al. 2009. J. Pharmacol Exp Ther. 328:371. PubMed
Wang X, et al. 2014. Int J Cardiol. 174:688. PubMed
Sautto GA, et al. 2015. Gut. PubMed
Kulkarni O, et al. 2009. J Pharmacol Exp Ther. 328:371. PubMed
Davis A, et al. 2008. J Immunol. 180:6923. PubMed
Qiu J, et al. 2010. Appl Envir Microbiol. 76:5846. PubMed
Wang X, et al. 2014. Int J Cardiol. 174:6888. PubMed
Sautto G, et al. 2015. Gut. . PubMed
Grech D, et al. 2016. J Acupunct Meridian Stud. 9:234-241. PubMed
Jø nsson K, et al. 2017. Nat Commun. 8:14391. PubMed
Bikker R, et al. 2017. Cell Signal. 10.1016/j.cellsig.2017.06.009. PubMed
Kong B, et al. 2017. J Neuroinflammation. 10.1186/s12974-017-0961-z. PubMed

Standard Range

7.8-500 pg/mL

Materials Not Included

Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
A microplate reader capable of measuring absorbance at 450 nm
Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
Deionized (DI) water
Coating Buffer: 8.4 g NaHCO₃, 3.56 g Na₂CO₃, add DI H₂O to 1.0 L, pH to 9.5 (BioLegend Cat. No. 421701 is recommended.)
Assay Diluent: 10% Fetal Bovine Serum or 1% BSA in Phosphate-Buffered Saline (PBS) (BioLegend Cat. No. 421203 is recommended.)
PBS: 8.0 g NaCl, 1.16 g Na₂HPO₄, 0.2 g KH₂PO₄, 0.2 g KCl, add DI water to 1.0 L, pH to 7.4
Wash Buffer: Phosphate-Buffered Saline (PBS) + 0.05% Tween-20 (BioLegend Cat. No. 421601 is recommended.)
Wash bottle or automated microplate washer
TMB Substrate Solution – BioLegend Cat. No. 421101 is recommended.
Stop Solution (2 N H₂SO₄)
Log-Log graph paper or software for data analysis
Tubes to prepare standard dilutions
Timer
Absorbent paper

Kit Contents

Human TNF-α ELISA MAX™ Capture Antibody (200X)
Human TNF-α ELISA MAX™ Detection Antibody (200X)
Human TNF-α Standard
Avidin-HRP (1000X)

Cell Sources: Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Gene ID: 7124View all products for this Gene ID
UniProt: View information about TNF-alpha on UniProt.org