Human IL-6 ELISA MAX™ Standard 5 Plates
ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation
BioLegend’s ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend’s ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.
Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.
- Reactivity
- Human
- Application
ELISA
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- Application References
(PubMed link indicates BioLegend citation)
- Ishola Jr DA, et al. 2006. Kidney International 2006; doi:10.1038/sj.ki.5001629PubMed
- Kim I, et al. 2006. Blood doi:10.1182/blood-2006-04-014399
- Wimmer A, et al. 2006. Blood 108:3722.
- Inokuchi J, et al. 2009. Carcinogenesis. 30:1028. PubMed
- Hirari M, et al. 2011. Blood 117:500. PubMed
- Zhou J, et al. 2015. Cytokine. 71:89. PubMed
- Inokuchi J, et al. 2009. Carcinogenesis. 30:1082. PubMed
- Hirai M, et al. 2011. Blood. 117:500. PubMed
- Zhou J, et al. 2015. Cytokine. 71:89. PubMed
- Grech D, et al. 2016. J Acupunct Meridian Stud. 9:234-241. PubMed
- Kong B, et al. 2017. J Neuroinflammation. 10.1186/s12974-017-0961-z. PubMed
- Standard Range
- 7.8-500 pg/mL
- Materials Not Included
- Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
- A microplate reader capable of measuring absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
- Deionized (DI) water
- Coating Buffer: 8.4 g NaHCO3, 3.56 g Na2CO3, add DI H2O to 1.0 L, pH to 9.5 (BioLegend Cat. No. 421701 is recommended.)
- Assay Diluent: 10% Fetal Bovine Serum or 1% BSA in Phosphate-Buffered Saline (PBS) (BioLegend Cat. No. 421203 is recommended.)
- PBS: 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add DI water to 1.0 L, pH to 7.4
- Wash Buffer: Phosphate-Buffered Saline (PBS) + 0.05% Tween-20 (BioLegend Cat. No. 421601 is recommended.)
- Wash bottle or automated microplate washer
- TMB Substrate Solution – BioLegend Cat. No. 421101 is recommended.
- Stop Solution (2 N H2SO4)
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Absorbent paper
- Kit Contents
- Human IL-6 ELISA MAX™ Capture Antibody (200X)
- Human IL-6 ELISA MAX™ Detection Antibody (200X)
- Human IL-6 Standard
- Avidin-HRP (1000X)
- Cell Sources
- T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells
- Gene ID
- NA
- UniProt
- View information about IL-6 on UniProt.org
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