LEGEND MAX™ Human IFN-g ELISA Kit with Pre-coated Plates 5 Pre-coated Plates
Human IFN-γ (Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-γ, IFN-gamma, etc.) is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on its anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. Natural IFN-γ is a dimeric protein with subunits of 143 amino acids. IFN-γ is synthesized as a precursor protein of 166 amino acids including a secretory signal sequence of 23 amino acids. The protein is glycosylated.
BioLegend’s LEGEND MAX™ Human IFN-γ ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Human IFN-γ specific monoclonal antibody is pre-coated on a 96-well strip-well plate.
BioLegend’s LEGEND MAX™ Human IFN-γ ELISA Kit is specifically designed for the accurate quantitation of Human IFN-γ from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.
- Reactivity
- Human
- Application
ELISA
- Application References
(PubMed link indicates BioLegend citation)
- Saito T, et al. 2014. Biochem Biophys Res Commun. 456:799. PubMed
- Johnston LR, et al. 2015. Atherosclerosis. 239:283. PubMed
- Saito T, et al. 2014. Biochem Biophys Res Commun. 456:799. PubMed
- Johnston L, et al. 2015. Atherosclerosis. 239:283. PubMed
- Henault J, et al. 2015. Nat Immunol. 17: 196-203. PubMed
- Sensitivity
- 5.6 pg/mL
- Standard Range
- 15.6-1,000 pg/mL
- Materials Not Included
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Plate Shaker
- Polypropylene vials
- Kit Contents
- Anti-Human IFN-γ Pre-coated 96-well Strip Microplate
- Human IFN-γ Dectection Antibody
- Human IFN-γ Standard
- Avidin-HRP A
- Assay Buffer A
- Wash Buffer (20X)
- Substrate Solution F
- Stop Solution
- Plate Sealers
- Cell Sources
- CD8+ and CD4+ T cells, NK cells
- Gene ID
- 3458View all products for this Gene ID
- UniProt
- View information about IFN-gamma on UniProt.org
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