Human TNF-a ELISA MAX™ Deluxe 20 Plates | Medical Supply Company

Human TNF-a ELISA MAX™ Deluxe 5 Plates

SKU: 430204 Categories: Deluxe Set, ELISA, Human, Sandwich Tag: Biolegend BV

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Human TNF-a ELISA MAX™ Deluxe 5 Plates

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend’s ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price!
BioLegend’s ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Reactivity

Human

Application

ELISA

As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.

Application References (PubMed link indicates BioLegend citation)

Subramanya S, et al. 2009. J. Virol. PubMed
Schyth BD, et al. 2012. Nucleic Acids Res. 40:463. PubMed.
Subramanya S, et al. 2010. J Virol. 84:2490. PubMed
Schyth B, et al. 2012. Nucleic Acids Res. 40:4653. PubMed
McIntyre B, et al. 2015. Innate Immunity. 21: 504 – 511. PubMed
XS R, et al. 2015. Diabetes. 64 90. PubMed
Naji A, et al. 2016. Sci Rep. 6: 26162. PubMed
Kunyanee C, et al. 2016. PLoS One. 11: 0160741. PubMed
Millrine D, et al. 2016. Proc Natl Acad Sci U S A. 113: 10625 – 10630. PubMed
Asakura M, et al. 2016. Sci Rep. 6:35633. PubMed
CarriÓn M, et al. 2016. J Leukoc Biol. 100(6):1385-1393. PubMed

Sensitivity

2 pg/ml

Standard Range

7.8-500 pg/mL

Materials Not Included

A microplate reader capable of measuring absorbance at 450 nm
Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
Deionized (DI) water
PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na₂HPO₄, 0.2 g KH₂PO₄, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
Wash bottle or automated microplate washer
Stop Solution (e.g. 2N H₂SO₄) Biolegend Cat. No. 423001 is recommended
Log-Log graph paper or software for data analysis
Tubes to prepare standard dilutions
Timer
Plate Sealer
Absorbent paper

Kit Contents

Human TNF-α ELISA MAX™ Capture Antibody (200X)
Human TNF-α ELISA MAX™ Detection Antibody (200X)
Human TNF-α Standard
Avidin-HRP (1000X)
Substrate Solution A
Substrate Solution B
Coating Buffer A (5X)
Assay Diluent A (5X)
NUNC Maxisorp™ 96 MicroWell Plates

Cell Sources: Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Gene ID: 7124View all products for this Gene ID
UniProt: View information about TNF-alpha on UniProt.org