LEGEND MAX™ Human IL-27 ELISA Kit with Pre-coated Plates 5 Pre-coated Plates
Human IL-27 is a member of the IL-12 cytokine family and is a heterodimeric protein composed of EBI3 (Epstein-Barr virus-induced gene 3) and p28 protein. It is expressed on naïve CD4+ T cells and natural killer cells. IL-27 is produced by antigen-presenting cells to induce proliferation of naive CD4+ T cells and activates human monocytes via STAT1 in addition to suppressing IL-10 production. IL-27 mediates its biological effects through the orphan cytokine receptor WSX-1/TCCR. IL-27 strongly synergizes with IL-12 to support IFN-γ production and differentiation of naive CD4+ T cells into T helper type.
BioLegend’s LEGEND MAX™ Human IL-27 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Human IL-27 specific monoclonal antibody is pre-coated on a 96-well strip-well plate.
BioLegend’s LEGEND MAX™ Human IL-27 ELISA Kit is specifically designed for the accurate quantitation of Human IL-27 from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.
- Reactivity
- Human
- Application
ELISA
- Application References
(PubMed link indicates BioLegend citation)
- Liu L, et al. 2012. J Biol Chem. 287:11899.PubMed
- He L, et al. 2014. PLoS One. 9:96792. PubMed
- Liu L, et al. 2012. J Biol Chem. 287:11899. PubMed
- He L, et al. 2014. PLoS One. 9:96792. PubMed
- Chang K, et al. 2017. Cell Death Dis. 10.1038/cddis.2017.95. PubMed
- Sensitivity
- 11 pg/mL
- Standard Range
- 0.25-16 ng/mL
- Materials Not Included
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Plate Shaker
- Polypropylene vials
- Kit Contents
- Anti-Human IL-27 Pre-coated 96-well Strip Microplate
- Human IL-27 Dectection Antibody
- Human IL-27 Standard
- Avidin-HRP A
- Assay Buffer A
- Wash Buffer (20X)
- Substrate Solution F
- Stop Solution
- Plate Sealers
- Cell Sources
- Naïve CD4+ T cells and natural killer cells
- Gene ID
- 246778View all products for this Gene ID
- UniProt
- View information about IL-27 on UniProt.org
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